Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Benjamin Hsu
Date created: 2015-09-16 11:00:00
Date modified: 2015-09-18 01:00:50
AANAT Expression
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1745002_sequence (Version 1) |
Description
Expression and possibly secretion of enzyme AANAT (aralkylamine N-acetyltransferase) [ Homo sapiens (human) ]. AANAT catalyzes Serotonin into N-acetylserotonin, a Melatonin precursor, and it is the rate-limiting step in Melatonin production. (Zheng 2002) Melatonin (N-acetyl-5-methoxytryptamine) is resposible for regulating circadium rhythms such as the sleep cycle and seasonal behaviors such as color change and reproduction in animals.(Lincoln 2003) In humans, AANAT is secreted in the Pineal gland. This construct is His-tagged.Notes
One thing that was uncertain was whether the AANAT could be secreted after expression, and if it could be secreted, whether it could retain its activity or not. We had thought about adding secretion pathway signals such as the Tat and Sec signal to the construct, and we had also thought about using addition methods such as adding phage shock protein genes and Kil genes, but in the end we decided against it because it would be hard to assay. We resolved to put these other genes on separate constructs.Source
Homo Sapiens (Human)| Sequence Annotation | Location | Component / Role(s) |
| His Tag AANAT(human) Constitutive Promoter RBS | 684,713 61,684 1,36 37,61 | tag feature/tag feature/protein CDS feature/promoter promoter feature/rbs ribosome_entry_site |