Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Tushar Nichakawade
Date created: 2015-09-10 11:00:00
Date modified: 2015-09-11 12:20:04
TdT delta1-27 (N-terminal Truncated Variant of TdT)
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1746000_sequence (Version 1) |
Description
This DNA sequence is a truncation of full length bovine TdT that was originally designed by Repasky et. all in a Mutational Analysis of Terminal Deoxynucleotidyltransferase Mediated N-Nucleotide Addition in V(D)J Recombination. This deletion was of a putative nuclear localization sequence. This sequence was demonstrated to show 80% catalytic activity relative to full length, untruncated, TdT in 293T cells.Notes
This DNA sequence was based on a truncated variant of bovine TdT which was then codon optimized for optimal expression in E. Coli, and chemically synthesized.Source
This DNA sequence was chemically synthesized and codon optimized for expression in E. Coli.| Sequence Annotation | Location | Component / Role(s) |
| NdeI cloning site Start Site Stop Site TdT delta1-27 | 1,6 3,6 1485,1491 7,1484 | feature/misc sequence_feature feature/start start_codon stop_codon feature/stop CDS feature/cds |