Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Christopher Lastihenos
Date created: 2015-09-10 11:00:00
Date modified: 2015-09-11 12:57:22
TdT delta26-143 (Truncated TdT Variant)
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1746001_sequence (Version 1) |
Description
This DNA sequence is a mutated variant of a sequence coding for the bovine terminal deoxytransferase (TdT) enzyme. The sequence was originally desinged by Repasky et. all in a Mutational Analysis of Terminal Deoxynucleotidyltransferase Mediated N-Nucleotide Addition in V(D)J Recombination. It includes a deletion of the 26th to 143rd amino acids of the naturally occurring TdT protein. It shows 55% catalytic activity relative to full length TdT in 293T cells. It is intended to be used for adding nucleotides to the 3 prime end of a DNA sequence.Notes
This DNA sequence was based on a truncated variant of bovine TdT which was then codon optimized for optimal expression in E. Coli, and chemically synthesized.Source
This DNA sequence was chemically synthesized and codon optimized for expression in E. Coli.| Sequence Annotation | Location | Component / Role(s) |
| Start Codon NdeI Site Protein coding sequence stop codon | 4,6 1,6 3,1211 1212,1214 | feature/start start_codon feature/misc sequence_feature feature/cds CDS stop_codon feature/stop |