Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Tim_Weenink
Date created: 2009-10-10 11:00:00
Date modified: 2015-05-08 01:10:59
Inducible Self Destructive Plasmid + inducible GFP-LVA reporter
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_K175043_sequence (Version 1) |
Description
Inducible Self Destructive Plasmid, consisting of IPTG inducible I-SceI homing endonuclease + inducible GFP-LVA reporter gene with an I-SceI restiction site integrated into the sequence between the promoter and the RBS. And an additional one between the GFP coding sequence and the TetR generator sequence.Notes
Initial design of the Self Destructive Plasmid. However, expression of the reporter gene did not seem to work after induction with anhydrotetracyclin.Source
See basic parts| Access | Instance | Definition |
| public public | BBa_K175040 BBa_K175042 | BBa_K175040 BBa_K175042 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K175040 BBa_K175042 | 1,1214 1223,3066 | BBa_K175040 BBa_K175042 |