BBa_K175040

BBa_K175040 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K175040

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Tim_Weenink

Date created: 2009-10-10 11:00:00

Date modified: 2015-05-08 01:10:59

I-SceI-LVA under p(LacI) + p(tetR) + I-SceI RS

Details

• Types: DnaRegion
• Roles: Intermediate; engineered_region
• Sequences: BBa_K175040_sequence (Version 1)

Description

I-SceI under a LacI controlled promoter (inducible with IPTG) followed by a p(tetR) and the I-SceI restriction site

Notes

construction intermediate in the creation of a Self Destructive Plasmid

Source

see basic parts

Components

Access	Instance	Definition
public	BBa_R0010	LacI
public	BBa_B0030	BBa_B0030
public	BBa_K142200	BBa_K142200
public	BBa_B0010	BBa_B0010
public	BBa_B0012	BBa_B0012
public	BBa_R0040	p(tetR)
public	BBa_K175027	I-SceI RS

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0010	1,200	LacI
BBa_B0030	209,223	BBa_B0030
BBa_K142200	230,977	BBa_K142200
BBa_B0010	986,1065	BBa_B0010
BBa_B0012	1074,1114	BBa_B0012
BBa_R0040	1123,1176	p(tetR)
BBa_K175027	1185,1214	I-SceI RS

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K175040/1

Member of these Collections

• iGEM Parts Registry