BBa_K175045

BBa_K175045 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K175045
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Tim_Weenink
Date created: 2009-10-10 11:00:00
Date modified: 2015-05-08 01:11:00

Self Destructive Plasmid with GFP-LVA reporter



    • Details

    Types
    DnaRegion

    Roles
    Device

    engineered_region

    Sequences BBa_K175045_sequence (Version 1)

    Description

    Final design of the Self Destructive Plasmid, consisting of a IPTG inducible I-SceI homing endonuclease, followed by its recognition site, followed by a anhydroTetracyclin (aTc) inducible GFP-LVA reporter gene (and a generator for TetR)

    Notes

    The restiction site was included only once and outside of any coding region, as the other format was shown to not work.

    Source

    Assembled in Delft

    Access Instance Definition
    public
    public
    		BBa_K175041
    BBa_K175044
    		BBa_K175041
    BBa_K175044

    Sequence Annotation Location Component / Role(s)
    BBa_K175041
    BBa_K175044
    		1,1114
    1123,3028
    		BBa_K175041
    BBa_K175044

    igem#experience
    Issues
     
    igem#sampleStatus
    It's complicated
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K175045/1
     

    Attachments

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