BBa_K1751003

BBa_K1751003 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1751003

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Tianlai Xia

Date created: 2015-08-15 11:00:00

Date modified: 2015-08-19 05:08:40

Promotor-RBS+RgPAL1

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K1751003_sequence (Version 1)

Description

Promotor-RBS:This part contains a constitutive promoter and strong RBS. The strength of this RBS is considered relative to BBa_B0031, BBa_B0032, BBa_B0033 and BBa_B0034. While the promotor is a member of a family of constitutive promotor parts labelled J23100 through J23119.
RgPAL1:The gene RgPAL1 can express Phenylalanine ammonia lyase（PAL）, which is an enzyme that catalyzes a reaction converting L-phenylalanine to ammonia and trans-cinnamic acid.PAL is the first and committed step in the phenyl propanoid pathway and is therefore involved in the biosynthesis of the polyphenol compounds. In our project, PAL plays an important role in L-phenylalanine???s deaminization to produce trans-cinnamic acid.
Terminator ：Using primers VR/VF2 to PCR B0010&B0012 will result in excess bands.

Notes

Promotor-RBS+RgPAL1+terminator

Source

iGEM's database and Rehmannia glutinosa Genome

Components

Access	Instance	Definition
public	BBa_K847100	BBa_K847100
public	BBa_K1751000	BBa_K1751000

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K847100	1,58	BBa_K847100
BBa_K1751000	65,2191	BBa_K1751000

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Planning
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1751003/1

Member of these Collections

• iGEM Parts Registry