Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Team Bielefeld-CeBiTec 2015
Date created: 2015-08-17 11:00:00
Date modified: 2015-09-19 01:12:55
Translation enhancing 5-UTR + sfGFP under control of T7 promoter
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_K1758102_sequence (Version 1) |
Description
sfGFP (BBa_I746916) under control of the T7 promoter (BBa_I719005). The part includes a translation enhancing sequence, 5'-UTR, which improves the binding of the mRNA to the ribosome (Olins et al. 1989) and is especially helpful in in vitro cell free protein synthesis. The 5'-UTR sequence contains a spacer of 10 adenine bases which has been shown to further enhance translation efficiency (Takahashi et al. 2013). The part also contains a double terminator made of B0010 and B0012.Notes
Lentini et al. 2013 showed that the scar which is created by standard biobrick assembly is disadvantageous for in vitro translation when occuring between RBS and start codon.| Access | Instance | Definition |
| public public | BBa_I719005 BBa_K1758101 | pT7 BBa_K1758101 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_I719005 BBa_K1758101 | 1,23 24,927 | pT7 BBa_K1758101 |