Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Team Bielefeld-CeBiTec 2015
Date created: 2015-08-12 11:00:00
Date modified: 2015-09-18 07:05:34
RNase E regulating protein RraA
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1758120_sequence (Version 1) |
Description
The product of the RraA-gene has been reported to interact with RNase E from E. coli and to alternate its activity. Airen showed that if RraA-protein is added to a cell free protein synthesis reaction, the productivity raises about 30% (Airen, 2011). He postulated that activity of RNase E in the reaction is lowered due to the interaction with RraA-protein, therefore mRNA-levels are stabilized. RNase E is brought to the in vitro reaction by E. coli cell extract itself.Notes
Amplification with Gibson-primer:*Amplification of regulator of ribonuclease activity A (RraA):
Forward primer RraA
5'-ATGAAATACGATACTTCCGAGCTTTG-3'
Reverse primer RraA
5'-TCATTCAATATCCAGCGGATCTTC-3'
*Amplification of the pSB1C3-Backbone:
Forward primer pSB1C3 RraA(43 bp)
5'-TCAGAAGATCCGCTGGATATTGAATGATACTAGTAGCGGCCGCTGCAG-3'
Reverse primer pSB1C3 RraA(43 bp)
5'-TTCTTCCAGTTGTTCCGGGTTTGCCATCTCTAGAAGCGGCCGCGAATTCC-3'
Ligation via Gibson-Assembly.
Source
RraA was obtained via PCR on E. coli K12 genomic DNA.| Sequence Annotation | Location | Component / Role(s) |
| rraA (menG) | 1,486 | feature/cds CDS |