Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Team Bielefeld-CeBiTec 2015
Date created: 2015-08-28 11:00:00
Date modified: 2015-09-18 07:22:27
His-tagged lac repressor fused to superfolder GFP under the control of the T7 promoter
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_K1758202_sequence (Version 1) |
Description
Uses T7 promotor and a strong rbs (BBa_K525998), the lac repressor linked with superfolding GFP (sfGFP) with a His-Tag. By linking sfGFP we want to detect bound protein via fluorescence.Notes
This is a composite part which enables a high protein expression and an easy purification of lacI fused to superfolding GFP (sfGFP). The green fluorescence of the culture is a sign for protein expression.Source
We clone sfGFP (Bba_I746916) via Gibson Assembly in the device K1758201.| Sequence Annotation | Location | Component / Role(s) |
| lacI BBa_K525998 RBS T7 promoter His-Tag BBa_B0034 sfGFP | 39,1127 1,32 24,27 1,23 1854,1877 21,32 1134,1853 | CDS feature/cds feature/BioBrick engineered_region ribosome_entry_site feature/rbs promoter feature/promoter feature/tag tag feature/BioBrick engineered_region CDS feature/cds |