BBa_K1758376

BBa_K1758376 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1758376

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Team Bielefeld-CeBiTec 2015

Date created: 2015-08-31 11:00:00

Date modified: 2015-09-19 01:22:36

GHB / GBL biosensor - reporter part

Details

• Types: DnaRegion
• Roles: Generator; engineered_region
• Sequences: BBa_K1758376_sequence (Version 1)

Description

This part contains all features from BBa_K1758102 plus the blc-operator site described in Pan et al. 2013. BlcR (see http://parts.igem.org/Part:BBa_K1758370 K1758370]) can bind to the operator site.

Notes

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Source

This part was assembled via PCR with Gibson primers on BBa_K1758102

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
T7 promoter	1,22	feature/promoter, promoter
Blc operator	23,63	operator, feature/operator
5' -UTR	64,109	sequence_feature, feature/misc
K1758100	64,109	feature/BioBrick, engineered_region
K1758101	64,966	feature/BioBrick, engineered_region
sfGFP	110,829	CDS, feature/cds
B0010	838,917	engineered_region, feature/BioBrick
B0012	926,966	feature/BioBrick, engineered_region
silent mutation	691,691	sequence_alteration, feature/mutation

Other Properties

• igem#experience: Works
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1758376/1

Member of these Collections

• iGEM 2018 Distribution Plate 7
• iGEM 2018 Distribution Plate 7 Well 13I
• iGEM 2018 Part Library
• iGEM 2018 Plasmid pSB1C3
• iGEM 2019 Distribution Plate 5
• iGEM 2019 Distribution Plate 5 Well 13J
• iGEM 2019 Part Library
• iGEM 2019 Plasmid pSB1C3
• iGEM Parts Registry