Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Team Bielefeld-CeBiTec 2015
Date created: 2015-08-21 11:00:00
Date modified: 2015-09-18 05:00:27
His-tagged E. coli tryptophanase under control of the T7 promoter
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1758390_sequence (Version 1) |
Description
This part can be used for expression and purification of the E. coli tryptophanase.A tryptophanase catalyzes the reaction of L-tryptophan and water to indole, pyruvate, and NH3.
Notes
This part was obtained as linear gene synthesis and cloned into pSB1C3 via Gibson Assembly.Source
This part was obtained by gene synthesis.| Sequence Annotation | Location | Component / Role(s) |
| his-tag stop tryptophanase T7 promoter + RBS | 1454,1471 1472,1474 41,1453 1,32 | feature/tag tag feature/stop stop_codon CDS feature/cds promoter feature/promoter |