Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Hao Jiang, Danqian Liu, Chao Li
Date created: 2009-06-15 11:00:00
Date modified: 2015-05-08 01:11:01
pSB1A3 with the suffix of J61002 (mRFP)
| Types | DnaRegion |
| Roles | Plasmid_Backbone plasmid_vector |
| Sequences | BBa_K176007_sequence (Version 1) |
Description
It's the same plasmid backbone of pSB1A3, but sequence between SpeI and PstI in the suffix are from plasmid backbon J61002.SpeI-RBS-mRFP-Terminator-PstI-pSB1A3-prefix
Notes
This plasmid backbone is generated by cutting a part in J61002 with X+P, and ligating it into pSB1A3 cutted with X+P.Source
pSB1A3 J61002| Sequence Annotation | Location | Component / Role(s) |
| B0034 SpeI RBS mRFP B0015 rrnB terminator NotI PstI a part of J61002 a part of pSB1A3 prefix deletion of ac in pSB1A3 t->c in pSB1A3 g->a in pSB1A3 | 9,20 2,7 9,20 27,707 741,869 752,795 878,885 885,890 1,890 878,3026 3005,3026 902,903 1149,1149 1584,1584 | engineered_region feature/BioBrick sequence_feature feature/dna ribosome_entry_site feature/rbs feature/cds CDS engineered_region feature/BioBrick stem_loop feature/stem_loop feature/dna sequence_feature feature/dna sequence_feature engineered_region feature/BioBrick engineered_region feature/BioBrick engineered_region feature/BioBrick sequence_alteration feature/mutation feature/mutation sequence_alteration sequence_alteration feature/mutation |