Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Danqian Liu, Hao Jiang
Date created: 2009-08-09 11:00:00
Date modified: 2015-05-08 01:11:02
Cell density controller: PoPS->luxI-LVA+pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB(weak RBS)
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_K176091_sequence (Version 1) |
Description
Cell Death = (Cell Density * Input PoPS) + Input AHLHigh Input PoPS -> Low Steady State Cell Density
High Input AHL -> Low Steady State Cell Density
Notes
Input1: PoPSInput2: AHL
Output1: AHL
Output2: Cell Density
Design:
Input1 PoPS generates LuxI-LVA, LuxI-LVA generates Output1 AHL, the concentration of AHL in the medium depends on both LuxI-LVA level per cell and cell density.
pCon 0.70(J23101) generates LuxR.
pLux/Tet(K176000) generates ccdB to kill the cell, activated by LuxR and (Output1 AHL generated by LuxI-LVA + Input2 AHL), repressed by TetR and no aTc.
Source
K176080 K176063| Access | Instance | Definition |
| public public | BBa_K176080 BBa_K176063 | BBa_K176080 BBa_K176063 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K176080 BBa_K176063 | 1,1865 1874,2510 | BBa_K176080 BBa_K176063 |