BBa_K176091

BBa_K176091 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K176091
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Danqian Liu, Hao Jiang
Date created: 2009-08-09 11:00:00
Date modified: 2015-05-08 01:11:02

Cell density controller: PoPS->luxI-LVA+pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB(weak RBS)



Types
DnaRegion

Roles
Device

engineered_region

Sequences BBa_K176091_sequence (Version 1)

Description

Cell Death = (Cell Density * Input PoPS) + Input AHL

High Input PoPS -> Low Steady State Cell Density

High Input AHL -> Low Steady State Cell Density

Notes

Input1: PoPS

Input2: AHL

Output1: AHL

Output2: Cell Density

Design:

Input1 PoPS generates LuxI-LVA, LuxI-LVA generates Output1 AHL, the concentration of AHL in the medium depends on both LuxI-LVA level per cell and cell density.

pCon 0.70(J23101) generates LuxR.

pLux/Tet(K176000) generates ccdB to kill the cell, activated by LuxR and (Output1 AHL generated by LuxI-LVA + Input2 AHL), repressed by TetR and no aTc.

Source

K176080 K176063

igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K176091/1