| Types | DnaRegion
|
| Roles | Coding
CDS
|
| Sequences | BBa_K1761000_sequence (Version 1)
|
Description
OmpX is an outer membrane protein with the C- and N-termini in the intracellulair domain. To be able to use OmpX as a scaffold, a non-natural amino acid needs to be introduced. This can be done by implementing the amber stop codon TAG in one of the loops of OmpX via a mutation. With a specific tRNA an azide-functionalized amino acid can be built in, which can be used for the SPAAC click chemistry reaction with DBCO functionalized groups.
Notes
Codon optimalization and deleting restriction sites used for biobricking and classical cloning.
Source
An outer membrane protein naturally occuring in wildtype E.coli.
