BBa_K176115

BBa_K176115 Version 1

Component

This part has been discontinued.

Source: http://parts.igem.org/Part:BBa_K176115

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Danqian Liu, Zongxiao He, Hao Jiang

Date created: 2009-10-07 11:00:00

Date modified: 2015-07-09 01:17:48

Cell density controller: PoPS->luxI-LVA+pCon 0.70->luxR+pLux/Tet->ccdB(weak RBS)

Details

• Types: DnaRegion
• Roles: Device; engineered_region
• Sequences: BBa_K176115_sequence (Version 1)

Description

Cell Death = (Cell Density * Input PoPS) + Input AHL

High Input PoPS -> Low Steady State Cell Density

High Input AHL -> Low Steady State Cell Density

Notes

Input1: PoPS

Input2: AHL

Output1: AHL

Output2: Cell Density

Design:

Input1 PoPS generates LuxI-LVA, LuxI-LVA generates Output1 AHL, the concentration of AHL in the medium depends on both LuxI-LVA level per cell and cell density.

pCon 0.70(J23101) generates LuxR.

pLux/Tet(K176000) generates ccdB to kill the cell, activated by LuxR and (Output1 AHL generated by LuxI-LVA + Input2 AHL), repressed by TetR and no aTc.

Source

K176080 K176062

Components

Access	Instance	Definition
public	BBa_K176080	BBa_K176080
public	BBa_K176062	BBa_K176062

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K176080	1,1865	BBa_K176080
BBa_K176062	1874,2336	BBa_K176062

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Deleted
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K176115/1

Member of these Collections

• iGEM Parts Registry