BBa_K176159

BBa_K176159 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K176159
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Danqian Liu, Zongxiao He, Hao Jiang
Date created: 2009-09-21 11:00:00
Date modified: 2015-05-08 01:11:03

PoPS->RBS+ccdB-LVA->PoPS



Types
DnaRegion

Roles
engineered_region

Translational_Unit

Sequences BBa_K176159_sequence (Version 1)

Description

RBS + ccdB-LVA

Notes

strong RBS + ccdB-LVA coding

Source

PCR from K176010.

Forward Primer: VF2(G00100)

Reverse Primer: GTTT ACTAGT A TTATTA AGC TAC TAA AGC GTA GTT TTC GTC GTT TGC AGC tattccccagaacatcaggtta

Digest with EcoRI and SpeI, ligate into pSB1A3.

Access Instance Definition
public
public
BBa_B0034
BBa_K176157
BBa_B0034
ccdB-LVA
igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K176159/1