AID

BBa_K176190 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K176190

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Danqian Liu, Zongxiao He, Hao Jiang

Date created: 2009-10-09 11:00:00

Date modified: 2015-05-08 01:11:04

Activation-Induced (Cytidine) Deaminase (standard version of K103001)

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K176190_sequence (Version 1)

Description

AID is an enzyme which can increase mutation rate in vivo by C->U conversion on DNA.

[[Part:BBa_K103001|K103001]] is the coding sequence of AID with NcoI site before start codon. This part K176190 is a standard AID coding sequence starts with ATG and ends with TAATAA.

Reference for Biology and Usage:

http://en.wikipedia.org/wiki/Activation-Induced_%28Cytidine%29_Deaminase

http://2009.igem.org/Team:USTC

http://2008.igem.org/Team:Warsaw

http://2008.igem.org/Team:Peking_University

http://www.ccbi.cam.ac.uk/iGEM2007/index.php/Gamma_-_automatic_directed_evolution

Notes

Standard AID coding sequence starts with ATG and ends with TAATAA.

Source

PCR from [[Part:BBa_K103001|K103001]].

Forward Primer: GTTTC T TCTAGa tg gacagcctcttgatgaacc

Reverse Primer: GTTTC CTGCA G CGGCCGC T ACTAGT A TTATTa aagtcccaaagtacgaaatg

Digest with XbaI and PstI, ligate into pSB1A3.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Start	1,3	start_codon, feature/start
Stop	595,600	feature/stop, stop_codon
Activation-Induced (Cytidine) Deaminase	1,594	CDS, feature/cds

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K176190/1

Member of these Collections

• iGEM Parts Registry