BBa_K176241

BBa_K176241 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K176241
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Danqian Liu, Hao Jiang
Date created: 2009-10-13 11:00:00
Date modified: 2015-05-08 01:11:05

High input score automatic selector (with reporter): PoPS->GFP->K176238



Types
DnaRegion

Roles
Device

engineered_region

Sequences BBa_K176241_sequence (Version 1)

Description

PoPS->GFP->luxI-LVA->tetR-LVA + pCon 0.01->luxI-LVA + pCon 0.70->luxR + pLux/Tet->lacZalpha-ccdB(weak RBS)

Cell Death = (Cell Density * (pCon 0.01 + Population Average Input PoPS) + Input AHL) / (1 + Input PoPS)

Cells with relative high input score in the population will be selected, cells with relative low input score will die.

The first luxI-LVA is used to calculate average score in the population.

GFP is input score reporter.

Notes

PoPS->GFP->luxI-LVA->tetR-LVA + pCon 0.01->luxI-LVA + pCon 0.70->luxR + pLux/Tet->lacZalpha-ccdB(weak RBS)

Input1: PoPS

Input2: AHL

Output1: AHL

Output2: Cell Density

Output3: GFP

Design:

Input1 PoPS generates Output3 GFP, LuxI-LVA and TetR-LVA.

pCon 0.01(J23103) also generates LuxI-LVA, LuxI-LVA generates Output1 AHL, the concentration of AHL in the medium depends on both LuxI-LVA level per cell and cell density.

pCon 0.70(J23101) generates LuxR.

pLux/Tet(K176000) generates lacZalpha-ccdB (weak RBS) to kill the cell, activated by LuxR and (Output1 AHL generated by LuxI-LVA + Input2 AHL), repressed by TetR-LVA and no aTc.

Source

K176106 K176227

igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K176241/1