BBa_K176247

BBa_K176247 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K176247
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Chao Li, Hao Jiang
Date created: 2009-10-14 11:00:00
Date modified: 2015-05-08 01:11:05

High input score automatic selector without lethal device (with reporter): PoPS->mRFP->K176240->PoPS



Types
DnaRegion

Roles
engineered_region

Device

Sequences BBa_K176247_sequence (Version 1)

Description

PoPS->mRFP->luxI-LVA->tetR-LVA + pCon 0.15->luxI-LVA + pCon 0.70->luxR + pLux/Tet->PoPS

Cell Death = (Cell Density * (pCon 0.15 + Population Average Input PoPS) + Input AHL) / (1 + Input PoPS)

If output is a lethal device, cells with relative high input score in the population will be selected, cells with relative low input score will die.

The first luxI-LVA is used to calculate average score in the population.

mRFP is input score reporter.

Notes

PoPS->mRFP->luxI-LVA->tetR-LVA + pCon 0.15->luxI-LVA + pCon 0.70->luxR + pLux/Tet->PoPS

Input1: PoPS

Input2: AHL

Output1: AHL

Output2: PoPS

Output3: mRFP

Design:

Input1 PoPS generates Output3 mRFP, LuxI-LVA and TetR-LVA.

pCon 0.15(K176008) also generates LuxI-LVA, LuxI-LVA generates Output1 AHL, the concentration of AHL in the medium depends on both LuxI-LVA level per cell and cell density.

pCon 0.70(J23101) generates LuxR.

pLux/Tet(K176000) generates Output2 PoPS, activated by LuxR and (Output1 AHL generated by LuxI-LVA + Input2 AHL), repressed by TetR-LVA and no aTc.

Source

K176223 K176237

igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K176247/1