Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Vinson Lam
Date created: 2015-07-26 11:00:00
Date modified: 2015-09-16 10:43:11
Major Spidroin Protein 2 (MaSp2) Sequencing Core with AB Sticky Ends
| Types | DnaRegion |
| Roles | DNA sequence_feature |
| Sequences | BBa_K1763009_sequence (Version 1) |
Description
This part contains the core sequence of MaSp2 that has been assembled with a 5'-AGTT-3' overhang on the 5' end of the sequence and 3'-ACAG-5' overhang on the 3' end of the sequence. This part was designed for use with ICA, along with parts BBa_K1763003 and BBa_K1763004 as an efficient method to assemble multiple MaSp2 sequences together. This setup is to show that this particular set of sticky ends is suitable for ICA. Before using in ICA, this part should be digested with BsaI, a type IIs restriction enzyme, which cuts DNA outside of its recognition site.This part codes for identical protein primary sequence as BBa_K17630002, but has a different DNA sequence. This part is intended to anneal to a sequencing primer that will allow longer composite MaSp genes to be read.
Notes
The gene sequence for MaSp2 is particularly GC-rich, which may complicate PCR.Source
This part is derived from the MaSp2 gene found in the spider N. Clavipes.| Sequence Annotation | Location | Component / Role(s) |
| BsaI recognition site (forward) BsaI recognition site(reverse) A sticky end B sticky end MaSp2 Core Sequence SeqF Primer Binding Site SeqR Primer Binding Site | 1,5 114,119 7,10 109,112 10,108 47,64 47,64 | sequence_feature feature/misc sequence_feature feature/misc feature/misc sequence_feature feature/misc sequence_feature sequence_feature feature/dna feature/primer_binding primer_binding_site feature/primer_binding primer_binding_site |