BBa_K1763013

BBa_K1763013 Version 1

Component

This part has been discontinued.

Source: http://parts.igem.org/Part:BBa_K1763013

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Vinson Lam

Date created: 2015-07-26 11:00:00

Date modified: 2015-09-16 03:23:17

M2-3(1C3)

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K1763013_sequence (Version 1)

Description

This a gene sequence consisting of three MaSp2 pieces assembled together into the pSB1C3 vector using Iterative Capped Assembly. This part does not have any regulatory sites, and is intended to be cloned into an expression vector.

Notes

The gene sequence for MaSp2 is particularly GC-rich, which may complicate PCR.

Source

This part is derived from the MaSp2 gene found in the spider N. Clavipes.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
8-his tag	3,27	sequence_feature, feature/misc
M2-AB	36,137	feature/BioBrick, engineered_region
M2-BC	138,239	engineered_region, feature/BioBrick
M2-CA	240,341	feature/BioBrick, engineered_region

Other Properties

• igem#experience: None
• igem#status: Deleted
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1763013/1

Member of these Collections

• iGEM Parts Registry