Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Vinson Lam
Date created: 2015-09-14 11:00:00
Date modified: 2016-02-10 11:27:38
MaSp1-SeqAB2
| Types | DnaRegion |
| Roles | sequence_feature DNA |
| Sequences | BBa_K1763423_sequence (Version 1) |
Description
This part contains the core sequence of MaSp2 that has been assembled with a 5'-AGTT-3' overhang on the 5' end of the sequence and 3'-ACAG-5' overhang on the 3' end of the sequence. This part was designed for use with ICA, along with parts BBa_K1763003 and BBa_K1763004 as an efficient method to assemble multiple MaSp2 sequences together. This setup is to show that this particular set of sticky ends is suitable for ICA. Before using in ICA, this part should be digested with BsaI, a type IIs restriction enzyme, which cuts DNA outside of its recognition site.Sequence and Features
Notes
The gene sequence for MaSp2 is particularly GC-rich, which may complicate PCR.Source
This part is derived from the MaSp2 gene found in the spider N. Clavipes.| Sequence Annotation | Location | Component / Role(s) |
| BsaI recognition site (forward) BsaI recognition site (reverse) A sticky end B sticky end MaSp1 Core Sequence MaSp1 SeqF Primer MaSp1 SeqR Primer | 1,5 114,119 7,10 109,112 11,108 41,58 41,58 | sequence_feature feature/misc sequence_feature feature/misc feature/misc sequence_feature feature/misc sequence_feature feature/dna sequence_feature feature/primer_binding primer_binding_site feature/primer_binding primer_binding_site |