Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Pontus H??jer
Date created: 2015-09-12 11:00:00
Date modified: 2015-09-18 10:11:51
BAR construct 1
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1766009_sequence (Version 1) |
Description
BAR construct 1 was created in the hope of making a receptor able to signal binding to an antigen. By making a chimera between a histidine kinase (EnvZ) and a affibody molecule (Z:HER2:342) we would have a receptor capable of detecting the HER2 (human epidermal growth factor receptor) protein biomarker, commonly used for detecting breast cancer. The construct would use the EnvZ-OmpR two-component regulatory system to signal binding and activation of the histidine kinase. However successful signaling has not been documented.The construct has been studied for expression, pressence in membrane and affinity towards HER2.
Notes
The bacterial antigen receptor (BAR), construct 1, was created by replacing part of the periplasmic domain of EnvZ. Structure prediction of the periplasmic domain of EnvZ revealed a possible PAS domain with a Beta-sheet->Coil->Alpha-helix->Coil structure. This structure was replaced with the Z:HER2:342 affibody with documented affinity towards the human epidermal growth factor receptor (HER2) (REFERENCE).| Sequence Annotation | Location | Component / Role(s) |
| BAR construct 1 GGSSAAG linker V5 tag Z:HER2:342 | 1,1341 1342,1363 1364,1404 256,429 | CDS feature/cds feature/cds CDS feature/tag tag sequence_feature feature/misc |