Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Pontus H??jer
Date created: 2015-09-13 11:00:00
Date modified: 2015-09-16 01:43:51
BAR construct 2
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1766010_sequence (Version 1) |
Description
The bacterial antigen receptor (BAR) was created in the hope of making a receptor able to signal binding to an antigen. By making a chimera between a histidine kinase (EnvZ) and a affibody molecule (Z:HER2:342) we would have a receptor capable of detecting the HER2 (human epidermal growth factor receptor) protein biomarker, commonly used for detecting breast cancer. The construct would use the EnvZ-OmpR two-component regulatory system to signal binding and activation of the histidine kinase. However successful function of the receptor has not been fully documented.The construct has been studied for expression, pressence in membrane and affinity towards HER2.
Notes
The bacterial antigen receptor (BAR), construct 1, was created by| Sequence Annotation | Location | Component / Role(s) |
| BAR construct 2 GGSSAAG linker V5 tag Z:HER2:342 GSGGGGSG linker | 1,1365 1366,1387 1388,1431 256,429 430,453 | CDS feature/cds CDS feature/cds tag feature/tag sequence_feature feature/misc sequence_feature feature/misc |