BBa_K1767002

BBa_K1767002 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1767002
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: naseeb saida
Date created: 2015-09-15 11:00:00
Date modified: 2015-09-16 04:40:33

P(Lac)IQ RBS Aiia RBS LuxR ter ter luxpR RBS tetR ter ter



Types
DnaRegion

Roles
Composite

engineered_region

Sequences BBa_K1767002_sequence (Version 1)

Description

We designed and built a kill switch mechanism that is based on the activation of a lethal gene downstream of an inducible promoter that is activated over time.


The presented part represents the first and the second module of our designed genetic circuit.
The here presented BioBrick consists of a constitutive promoter (I14032) driving expression of Aiia and LuxR followed by a Lux promoter (R0062) , driving expression of TetR.


By using this part one can control the amount of producing TetR which means that at the beginning the system will produce TetR. After a certain amount of time thatdepends on the initial concentration of AHL , the system will stop producing TetR due to the absence of AHL that interacts with LuxR to form its active conformation.
The scheme below explains how this BioBrick works:



Notes

none

Source

iGEM registry

igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1767002/1