BBa_K1768003

BBa_K1768003 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1768003
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Gert Pietersen
Date created: 2015-09-13 11:00:00
Date modified: 2015-09-14 01:57:21

Recombinase invertible constitutive promoter 2



Types
DnaRegion

Roles
engineered_region

Inverter

Sequences BBa_K1768003_sequence (Version 1)

Description

A reverse compliment of a constitutive promoter flanked by Lox71 and Lox66 which are recognised by the Cre enzyme and facilitates a single round of recombination between these sites. Thus the direction of the promoter can be changed allowing expression of a different gene than in the initial state. This part is the same as part BBa_K1768001 but Lox71 is upstream of the promoter with a downstream lox66.

Notes

Lox71 is reversed relative to Lox66.
Spacer sequence from non-coding pUC19 are added between Lox sites.

Source

Lox66: BBa_I718016
Lox71: BBa_J23100
Constitutive promoter: BBa_J23100

igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1768003/1