Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Tianyu Tan
Date created: 2015-09-16 11:00:00
Date modified: 2015-09-17 11:06:34
yEGFP-tagged Mn peroxidase
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1772000_sequence (Version 1) |
Description
Manganese Peroxidase fusion protein gene flanked by two linker sequences, with a a GFP tag on the N-terminus and a yeast secretion tag on the C-terminus. A constitutive medium strength pCyc promoter followed by a yeast kozak sequence caps the N-terminus, and a yeast terminator sequence caps the C-terminus.Notes
Our goal was to synthesize lignin-degrading enzymes to be secreted out of yeast cells, which we used the secretion tag for. The fluorescence tag was merely implemented to simplify detection of successful secretion. To avoid time-consuming and low-efficiency standard assembly techniques, this construct was designed to be synthesized by IDT using two GBlocks(c), which were to be joined by Gibson Assembly. Linker sequences were implemented in order to minimize interaction between protein domains. A constitutive promoter was implemented due to the intended application (a bioreactor pretreatment process where a yeast containing this part would secrete enzymes to continuously degrade lignin).Source
Promoter: BBa_I766555Kozak sequence: BBa_J63003
yEGFP (yeast Enhanced Green Fluorescent Protein): NCBI Genbank accession U73901.1
Linker sequences: BBa_K243004
Manganese Peroxidase from Heterobasidion irregulare TC 32-1, NCBI Reference Sequence: NW_009258207.1
Secretion tag: Bba_K416003
Terminator: BBa_K392003
| Access | Instance | Definition |
| public public public public public public public | BBa_I766555 BBa_J63003 BBa_K243004 BBa_K1772001 BBa_K243004 BBa_K416003 BBa_K392003 | BBa_I766555 Kozak & st ShortLinke BBa_K1772001 ShortLinke BBa_K416003 BBa_K392003 |