BBa_K1772001

BBa_K1772001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1772001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Tianyu Tan

Date created: 2015-09-16 11:00:00

Date modified: 2015-09-19 10:25:06

Manganese Peroxidase

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K1772001_sequence (Version 1)

Description

Protein coding sequence for Manganese Peroxidase enzyme, sequence optimized for yeast.
Mechanism: oxidizing of Mn2+ ions into Mn3+ ions using an irreversible redox reaction with the aid of fungal chelators such as oxalic acid. This enzyme is specifically used by Heterobasidion irregulare TC 32-1, a variety of white rot fungus, to break down any wooden material in it???s path. Although the enzyme itself is not directly linked to lignin breakdown, the Mn3+ ions that it creates are then able to attack and oxidize organic molecules such as phenolic substrates or several lignin compounds. These lignin compounds are broken down into free radicals which quickly degrade due to their molecular instability.
Note: Calcium ions and heme-B are involved with the enzyme???s mechanism.

Notes

This gene sequence excludes introns (noncoding segments) present in the original gene from Heterobasidion irregulare TC 32-1. Sequence optimization was done using IDT's codon optimizer. Illegal restriction enzyme sites (under RFC 10 definition) were detected using NEBcutter2 and removed with silent mutations.

Source

Heterobasidion irregulare TC 32-1

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1772001/1

Member of these Collections

• iGEM Parts Registry