Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Xilin Jiang
Date created: 2015-09-12 11:00:00
Date modified: 2015-09-20 09:18:32
two mir-21 binding sites
| Types | DnaRegion |
| Roles | mature_transcript_region RNA |
| Sequences | BBa_K1777000_sequence (Version 1) |
Description
This part is composite of a Renilla luciferase and two mir-21 binding sites in the 3'-UTR. With this modification in the 3'-UTR, this luciferase can be as a reporter of mir-21. If this part is expressed in a cell with high level expression of mir-21, it will report a decreased Renilla luciferase activity.Notes
We use PCR to synthesize the mir-21 binding domain. In order to make sure of this reporter's efficiency, we synthesize two binding sites which are combined together.Source
Renilla luciferase ofthis part come from psiCHECK-2 Vector.| Sequence Annotation | Location | Component / Role(s) |
| miR21 binding site miR21 binding site | 6,26 45,65 | feature/binding non_covalent_binding_site feature/binding non_covalent_binding_site |