Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Xuhang Li
Date created: 2015-09-12 11:00:00
Date modified: 2015-09-19 11:42:57
flexible linker with SV40 NLS
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1777005_sequence (Version 1) |
Description
This is a 40-50A flexible separation for fusion domains with a SV40 NLS, and its codon is optimized for mammalian. It can be used to link different protein domains for fusion proteins locating in cell nucleus.Notes
The codon is optimized for mammalian, and we insert a NLS to promote locating in cell nucleus. Besides ,we lengthen this linker to provide better separation of two domains. At last we avoid tandem repeat sequence(like GGGSPKKKRKVGGGS), which will facilitate fusing this linker to your domains via overlap PCR.Source
This part is improved from BBa_K1486003.| Sequence Annotation | Location | Component / Role(s) |
| the first linker the second linker scar from 3A assembly SV40 NLS SV40 NLS natural flexible linker artificial GS linker natural flexible linker artificial GS linker | 3,59 68,124 60,67 24,44 89,109 3,23 45,59 68,88 110,124 | CDS feature/cds feature/cds CDS feature/scar feature/tag tag feature/tag tag sequence_feature feature/misc feature/misc sequence_feature feature/misc sequence_feature sequence_feature feature/misc |