| Types | DnaRegion
|
| Roles | Coding
CDS
|
| Sequences | BBa_K1777017_sequence (Version 1)
|
Description
This is a 40-50A flexible separation for fusion domains with a SV40 NLS, and its codon is optimized for mammalian. It can be used to link different protein domains for fusion proteins locating in cell nucleus.
Notes
The codon is optimized for mammalian, and we insert a NLS to promote locating in cell nucleus. Besides ,we lengthen this linker to provide better separation of two domains. At last we avoid tandem repeat sequence(like GGGSPKKKRKVGGGS), which will facilitate fusing this linker to your domains via overlap PCR.
Source
This part is improved from BBa_K1486003.
