BBa_K1778002

BBa_K1778002 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1778002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Lehua Jia

Date created: 2015-09-10 11:00:00

Date modified: 2015-09-11 09:27:48

TRE-CYC1TATA is a recombinant promoter, which is constructed in order to make the Tet-on system func

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K1778002_sequence (Version 1)

Description

TRE-CYC1TATA is a recombinant promoter, which is constructed in order to make the Tet-on system function in Pichia pastoris, which is connected in series by TRE promoter and CYC1TATA sequence. The recombinant promoter can be combined with the correct conformation of rtTA to form a regulation mechanism to ensure the validity of the switch.

Notes

When using the part in Pichia pastoris,we need to consider the design of overlapping extension PCR that will be involved in the two part series, TRE compact promoter and CYC1TATA sequence.

Source

TRE-CYC1TATA consists of two parts of the TRE compact promoter and CYC1TATA sequence.

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1778002/1

Member of these Collections

• iGEM Parts Registry