| Types | DnaRegion
|
| Roles | engineered_region
Composite
|
| Sequences | BBa_K1778003_sequence (Version 1)
|
Description
RtTA-flag is a regulatory protein that can regulate the binding of tetracycline analogues.On the N side, a flag tag is brought.The purpose of the design is to verify that the rtTA regulatory proteins are able to be stably expressed in yeast cells, and the role of the project is to be verified.
Notes
When using the part in Pichia pastoris, we need to consider whether the protein is stable in yeast cells and whether the formation of its conformation is effective to identify specific promoter, which requires the support of experiments and the paper.
Source
rtTA is from the commercial plasmid pTet-on,flag tags is commonly used detective tags.
