| Types | DnaRegion
|
| Roles | polypeptide_domain
Protein_Domain
|
| Sequences | BBa_K1778004_sequence (Version 1)
|
Description
rtTA is a regulatory protein which can combined with tetracycline analogues. Like the protein in Biological signaling pathways. When it combine with tetracycline analogues, it can change its conformation to activate the subsequent signal path. Under the action of constitutive promoter, the regulatory protein expresses stably. When it combine with tetracycline analogues, rtTA change its conformation to activate the promoter, then the report gene expresses.
Notes
When we use the part in pichia pastoris, we need to think that if the protein can exist stably in yeast cell and activate the promoter effectively. It need to be proved by experiment and references.
Source
rtTA is from the plasmid pTet-on, It was merged by reverse TetR and the transcriptional activation area of VP16.
