Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Guang Zhang
Date created: 2015-09-03 11:00:00
Date modified: 2015-09-04 06:24:42
Magnetosome mamCD operon and mms6 operon
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1779100_sequence (Version 1) |
Description
The mamCD operon(composed of the genes mamC, D, F, and G) and the mms6 operon(contains mms6, mmsF, mgr4070, mgr4071, and mgr4074)are involved in magnetite crystal shape and size. The deletion of both the mamCD and mms6 operons seriously affects both the morphology of the magnetite magnetosome crystals and the alignment of the magnetosomes.You can amplify the sequence derictly with PCR method if you want to use magnetosome in your project.Notes
First we found the two operon's sequences from ncbi.then we designed a pair of primers to amplify the DNA fragment of the two operons.When designing the primers,we designed a 20bp overlap and then we use the overlap to join them together with fusion PCR.Source
Magnetospirillum gryphiswaldense MSR-1 v2, complete genome| Sequence Annotation | Location | Component / Role(s) |
| MGR_4070 MGR_4071 mmsF mms6 MGR_4074 mamG mamF mamD mamC | 123,1472 1476,2519 2695,3068 3034,3489 3532,3804 4170,4423 4438,4773 4894,5838 5868,6245 | sequence_feature feature/misc feature/misc sequence_feature feature/cds CDS feature/cds CDS feature/misc sequence_feature feature/cds CDS feature/cds CDS CDS feature/cds feature/cds CDS |