BBa_K1779200

BBa_K1779200 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1779200

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Xingyang Qiu

Date created: 2015-09-02 11:00:00

Date modified: 2015-09-07 02:04:21

Integration of three genes, including mamW, RFP and laccase

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K1779200_sequence (Version 1)

Description

ntegration of three genes, including mamW, RFP and laccase.Where mamW gene is isolated from MTB bacilli obtained can be used as an anchor protein.RFP gene is extracted from iGEM official bio bricks, in this experiment, as a reporter gene expression can display fusion gene.Laccase gene is also obtained from the iGEM official bio bricks and paint as an oxidase enzyme, in this experiment, as the cathodic reaction main substance involved in the biofuel cell.

Notes

We designed primers with linker will mamW, RFP and laccase three genes linked by PCR of fusion.The mamW and RFP genes'stop codons have been removed.

Source

mamW gene is from MTB's genomic sequence.
RFP gene is obtained from the iGEM official bio bricks, which numbered BBa_E1010.
Laccase gene is obtained from the iGEM official bio bricks, which numbered BBa_K863005.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
mamaW	1,414	feature/cds, CDS
A to G	264,264	feature/mutation, sequence_alteration
G3S2 linker	415,450	sequence_feature, feature/misc
RFP	451,1125	feature/cds, CDS
G4S3 linker	1126,1170	feature/misc, sequence_feature
laccase	1171,2721	CDS, feature/cds

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1779200/1

Member of these Collections

• iGEM Parts Registry