Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Iowis Zhu
Date created: 2015-08-13 11:00:00
Date modified: 2015-09-25 09:08:37
Unstable RFP under Hok/Sok Regulation
| Types | DnaRegion |
| Roles | engineered_region Measurement |
| Sequences | BBa_K1783003_sequence (Version 1) |
Description
Produces unstable RFP under a constitutive promoter. This construct carries the hok-sok toxin-antitoxin (TA) plasmid maintenance system and was used to measure the ability of this alternative plasmid maintenance system to keep plasmids within the cell without the use of antibiotics.Notes
PCR of Hok-Sok component was difficult due to high inherent secondary structure of the gene sequences. DMSO was needed in order to break this structure.Source
See construction parts.| Access | Instance | Definition |
| public public | BBa_K1783001 BBa_K1783002 | BBa_K1783001 BBa_K1783002 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1783001 BBa_K1783002 | 1,580 581,1358 | BBa_K1783001 BBa_K1783002 |