Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Graeme Glaister
Date created: 2015-09-16 11:00:00
Date modified: 2015-09-17 09:44:15
Theophylline dependent ribozyme
| Types | DnaRegion |
| Roles | sequence_feature DNA |
| Sequences | BBa_K1791000_sequence (Version 1) |
Description
Theophylline dependent ribozyme with a spacer sequence (AU rich) flanking both sides of the part to assist in proper folding of the ribozyme. The ribozyme has been shown to induce cleave in vivo upon the addition of theophylline. However expressing it in vitro results in cleavage of the ribozyme both with and without the addition of theophylline.Notes
We have tested various temperatures, Magnesium and spermidine concentration under in vitro conditions but were unable to prevent the ribozmye from cleaving without the addition of theophylline. We will be investigating mutations to the theophylline ribozyme in order to optimize it for invitro conditions.Source
Adapted from Win and Smolke (2007). A modular and extensible RNA-based gene-regulatory platform for engineering cellular function. PNAS. 104(36):14283-8.| Sequence Annotation | Location | Component / Role(s) |
| Theophylline aptazyme | 12,108 | sequence_feature feature/misc |