Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nannan Xie
Date created: 2015-09-16 11:00:00
Date modified: 2015-09-18 02:35:42
promoter of Paenibacillus sp. WLY78 nif gene cluster
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_K1796001_sequence (Version 1) |
Description
The promoter of Paenibacillus sp. WLY78 nif gene cluster, We named it Pnif. Pnif is a σ70-dependent promoter located upstream of nifB. Pnif is in control of all the gene clusters of nitrogen fixation genome.Confirmation of nif promoter
We standardized the nif promoter of Paenibacillus sp. WLY78, and linked it to the upstream of 3 reporter genes (RFP reporter: BBa_K1357010, YFP reporter: BBa_E0430;amilCP reporter: BBa_K1357009) to confirm the reliability of this promoter. The results are shown in Figure 2.
A B C
http://2015.igem.org/wiki/images/b/b2/Confirmation_of_Pnif_AB.png
http://2015.igem.org/wiki/images/thumb/3/34/C-confirmation_of_Pnif.jpeg/622px-C-confirmation_of_Pnif.jpeg
Fig.4. Confirmation of nif promoter of Paenibacillus sp. WLY78. (A) Pnif+RFP reporter; (B) Pnif+YFP reporter;(C)Pnif+amilCP reporter.
The results confirmed the reliability of Pnif. The initiation of Pnif does not require inducement. Thus it is a effective constitutive promoter.