BBa_K1796011

BBa_K1796011 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1796011

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Nannan Xie

Date created: 2015-09-16 11:00:00

Date modified: 2015-09-17 08:46:04

nifN promoted from Paenibacillus sp. WLY78

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K1796011_sequence (Version 1)

Description

Function: scaffold gene of nitrogen fixation system

Notes

We abtained the sequences from genomes of Paenibacillus sp. WLY78. First, we promoted it ourselves（done by Nan Wang and Nannan Xie).Then we sent the sequences to synthesis, but unfortunately, striction enzyme cut sites were involved(three PstI) after they promoted it again. But the part can be assembled by gibson assembly,that is what we did.

Source

NifN is taken from genomic sequence of Paenibacillus sp.WLY78, we apply promotions on the sequences and synthesised it.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
nifN coding sequence	22,1434	CDS, feature/cds
unknown rbs	1,21	ribosome_entry_site, feature/rbs

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/myers
• synbiohub#ownedBy: user/james
• synbiohub#topLevel: BBa_K1796011/1

Member of these Collections

• iGEM Parts Registry