BBa_K180014

BBa_K180014 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K180014
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Richard Wilson, Stephanie Chambers
Date created: 2009-10-20 11:00:00
Date modified: 2015-05-08 01:11:06

Rock-paper-scissors - Paper primary plasmid



Types
DnaRegion

Roles
Composite

engineered_region

Sequences BBa_K180014_sequence (Version 1)

Description

Composite part, consisting of BBa_K180005 and BBa_K180015, but with BBa_K180005 reading along the reverse strand, and BBa_K180015 reading along the forward strand.

Forms the 'paper' primary plasmid in Team:Southampton's rock-paper-scissors project design.

Notes

BBa_K180005 reading along reverse strand, and BBa_K180015 reading along the forward strand to ensure the constitutive promoter BBa_J23119 (present in BBa_K180005) has no effect over GFP or RhlI expression in BBa_K180015.

Source

See relevant biobrick component parts (BBa_K180011 and BBa_K180012).

Sequence Annotation Location Component / Role(s)
K180005
K180015
B0015
C0062
luxR
B0034
conserved
B0034
conserved
B0034
conserved
J23119
-35
-10
K180000
lacIq
Ptac
E0040
GFPmut3b
C0170
rhlI
B0015
1,979
988,3952
1,129
163,918
169,918
2443,2454
2447,2450
3189,3200
3193,3196
925,936
929,932
945,979
975,979
951,956
988,2434
988,2069
2312,2339
2461,3180
2461,3180
3207,3815
3207,3815
3824,3952
feature/BioBrick engineered_region
engineered_region feature/BioBrick
engineered_region feature/BioBrick
engineered_region feature/BioBrick
CDS feature/cds
engineered_region feature/BioBrick
feature/conserved conserved_region
engineered_region feature/BioBrick
feature/conserved conserved_region
engineered_region feature/BioBrick
feature/conserved conserved_region
feature/BioBrick engineered_region
promoter feature/promoter
feature/promoter promoter
engineered_region feature/BioBrick
CDS feature/protein
promoter feature/promoter
engineered_region feature/BioBrick
CDS feature/protein
engineered_region feature/BioBrick
CDS feature/cds
engineered_region feature/BioBrick
igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K180014/1