Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Arnas Petrauskas
Date created: 2015-09-15 11:00:00
Date modified: 2015-09-16 10:34:22
DBR2 gene from Artemisia annua, 6 His tagged
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1808003_sequence (Version 1) |
Description
This is the coding region of the Dbr2 gene from Artemisia annua with added biobrick suffix, prefix and a 6 His tag in a pSB1C3 plasmid backbone. This part is an element of TCD iGEM 2015 set of parts for the synthesis of the anti-malarial drug precursor dihydroartemisinic acid in E. coli. The Dbr2 gene encodes the enzyme artemisinic aldehyde delta11(13)-reductase, which catalyses the conversion of artemisinic aldehyde to (11R)-dihydroartemisinic aldehyde with NADPH as the electron donor substrate. The product can be acted upon by the Artemisia annua aldehyde dehydrogenase 1 enzyme (see part BBa_K1808001) to give rise to dihydroartemisinic acid, which can be isolated and converted to the anti-malarial artemisinin. This part is codon optimised for BioBrick 3A assembly and expression in E. coli cells as well as protein purification.Notes
This part is BioBrick 3A assembly, E. coli codon and synthesis optimised. This part is 6 His tagged to allow protein purification.Source
Artemisia annua| Sequence Annotation | Location | Component / Role(s) |
| DBR2 6His tagged 6 His | 1,1182 1161,1179 | CDS feature/cds feature/tag tag |