| Types | DnaRegion
|
| Roles | CDS
Coding
|
| Sequences | BBa_K1808005_sequence (Version 1)
|
Description
This is the coding region of the ADH1 gene from Artemisia annua with added biobrick suffix and prefix in a pSB1C3 plasmid backbone with a 6 His tag. This part is an element of TCD iGEM 2015 set of parts for the synthesis of the anti-malarial drug precursor dihydroartemisinic acid in E. coli. The Dbr2 gene encodes the enzyme alcohol dehydrogenase 1, which catalyses the conversion of artemisinic alcohol to artemisinic aldehyde with NAD+ as the electron donor substrate. The product can be acted upon by the Artemisia annua DBR2 enzyme (see part BBa_K1808000) to give rise to dihydroartemisinic acid, which can be isolated and converted to the anti-malarial artemisinin. This part is codon optimised for BioBrick 3A assembly and expression in E. coli cells as well as protein purification.
Notes
This part is codon optimised for BioBrick 3A assembly and expression in E. coli cells as well as protein purification.
Source
Artemisia annua
