BBa_K1808006

BBa_K1808006 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1808006

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Remsha Afzal

Date created: 2015-09-15 11:00:00

Date modified: 2015-09-16 11:07:37

CYB5 HIS from Artemisia annua

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K1808006_sequence (Version 1)

Description

This is the coding region of the CYB5 HIS gene from Artemisia annua with added biobrick suffix and prefix in a pSB1C3 plasmid backbone and a 6 HIS TAG. This part is an element of TCD iGEM 2015 set of parts for the synthesis of the anti-malarial drug precursor dihydroartemisinic acid in E. coli. The CYB5 was used by our team to increase the efficiency of the rate limiting step of Cytochrome P450 which catalyses the conversion of amorphadiene to artemisinic alcohol to artemisinic aldehyde and finally to artemisinic acid.

Notes

his part is codon optimised for BioBrick 3A assembly and expression in E. coli cells as well as protein purification

Source

Artemisia annua

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
CYB5	1,429	CDS, feature/cds
6 HIS	409,426	tag, feature/tag

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1808006/1

Member of these Collections

• iGEM Parts Registry