Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Arnas Petrauskas
Date created: 2015-09-15 11:00:00
Date modified: 2015-09-16 11:26:10
CYP71AV1 from Artemisia annua
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1808007_sequence (Version 1) |
Description
This is the coding region of the CYP71AV1 gene from Artemisia annua with added biobrick suffix, prefix and a 6 His tag in a pSB1C3 plasmid backbone. This part is an element of TCD iGEM 2015 set of parts for the synthesis of the anti-malarial drug precursor dihydroartemisinic acid in E. coli. The CYP71AV1 gene encodes the enzyme Amorphadiene oxidase, which catalyses the conversion of amorpha-4,11-diene to artemisinic alcohol, subsequent conversion of the alcohol to artemisinic aldehyde and the subsequent and final conversion of the artemisinic aldehyde to artemisinic acid with NADPH as the substrate. Artemisinic acid and dihydroartemisinic acid can be extracted from E. coli cells through lysis and chemically converted to the anti-malarial artemisinin. The Amorphadiene oxidase enzyme is a memberofthe cytochrome p450 family of hemoproteins and is the rate limiting enzyme in the synthesis pathway for artemisinic acid developed by Amyris. This part is codon optimised for BioBrick 3A assembly and expression in E. coli cells as well as protein purification.Notes
This part is BioBrick 3A assembly, E. coli codon and synthesis optimised. This part is 6 His tagged to allow protein purification.Source
Artemisia annua| Sequence Annotation | Location | Component / Role(s) |
| CYP71AV1 6 His tagged 6 His | 1,1506 1485,1503 | CDS feature/cds feature/tag tag |