| Types | DnaRegion
|
| Roles | Coding
CDS
|
| Sequences | BBa_K1808009_sequence (Version 1)
|
Description
This is the coding region of the CPR gene from Artemisia annua with added biobrick suffix and prefix in a pSB1C3 plasmid backbone as well as a 6 his tagged component. This part is an element of TCD iGEM 2015 set of parts for the synthesis of the anti-malarial drug precursor dihydroartemisinic acid in E. coli. The CPR gene encodes the enzyme Cytochrome P450 reductase, which catalyses the conversion of NADPH back to NAD+ by transference of electron between Cytochrome P450. The product is used in the production of dihydroartemisinic acid, which can be isolated and converted to the anti-malarial artemisinin. This part is codon optimised for BioBrick 3A assembly and expression in E. coli cells as well as protein purification.
Notes
This part is codon optimised for BioBrick 3A assembly and expression in E. coli cells as well as protein purification.
Source
Artemisia annua
