Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Remsha Afzal
Date created: 2015-09-15 11:00:00
Date modified: 2015-09-16 12:13:35
ALDH1 regulated by the TAC promoter
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K1808011_sequence (Version 1) |
Description
This is a device that is an ALDH1 generator in a pSB1C3 plasmid backbone. It expressed ALDH1 if induced by IPTG and not repressed by tryptophan. This construct is an element of TCD iGEM 2015 for the synthesis of the anti-malarial drug precursor dihydroartemisinic acid in E. coli. The ALDH1 gene encodes the enzyme aldehyde dehydrogenase 1, which catalyses the conversion of (11R)-dihydroartemisinic aldehyde to dihydroartemisinic acid as well as the conversion of artemisinic aldehyde to artemisinic acid with NAD+ as the substrate. Artemisinic acid and dihydroartemisinic acid can be extracted from E. coli cells through lysis and chemically converted to the anti-malarial artemisinin. This construct was generated via 3A assembly and is optimised for expression in E. coli cells.Notes
This construct was generated via 3A assembly and is optimised for expression in E. coli cells.Source
Artemisia annua| Access | Instance | Definition |
| public public | BBa_K864400 BBa_S05306 | BBa_K864400 BBa_S05306 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K864400 BBa_S05306 | 1,61 70,1743 | BBa_K864400 BBa_S05306 |