BBa_K864400

BBa_K864400 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K864400
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Erik Lundin
Date created: 2012-09-24 11:00:00
Date modified: 2015-05-08 01:13:37

Ptac, trp & lac regulated promoter



    • Details

    Types
    DnaRegion

    Roles
    Regulatory

    promoter

    Sequences BBa_K864400_sequence (Version 1)

    Description

    The Ptac promoter is a functional hybrid promoter, derived from the trp and lac promoters, that are regulated by trp and lac [1]. This part also exist together with lacI, part [[Part:BBa_K180000|BBa_K180000]]

    [1] Proc. Natl. Acad. Sci. USA, Vol. 80, pp. 21-25

    Notes

    Oligos ordered from SigmaAldrich were annealed to form a double stranded DNA segment, ready to be ligated into any BioBrick backbone.

    Source

    Created from oligos ordered from SigmaAldrich.

    Oligo sequences:
    Ptac+
    AATTCGCGGCCGCTTCTAGAGGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTA

    Ptac-
    CTAGTAAATTGTTATCCGCTCACAATTCCACACATTATACGAGCCGATGATTAATTGTCAACAGCTCCTCTAGAAGCGGCCGCG

    Sequence Annotation Location Component / Role(s)
    Ptac
    -35
    -10
    lacO1 site
    		1,40
    7,12
    29,33
    36,61
    		feature/promoter promoter
    promoter feature/promoter
    feature/promoter promoter
    feature/operator operator

    igem#experience
    Works
     
    igem#partStatus
    Released HQ 2013
    igem#sampleStatus
    In stock
    igem#status
    Available
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K864400/1
     

    Attachments

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