Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Date created: 2015-09-12 11:00:00
Date modified: 2016-02-03 10:46:07
Melittin Optimized for E. coli with Mcherry
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1810002_sequence (Version 1) |
Description
Melittin is a antimicrobial peptide found in bee venom that works to cause cell lysis by forming a pore in the membrane of harmful bacteria. While the 2014 Stony Brook iGEM team was able to successful clone the melittin gene into E. coli, expression was inconsistent. The 2015 Stony Brook iGEM team worked to correct this by optimizing the codons for an E. coli cell. Both the optimized and regular versions of melittin were fused with a mCherry tag in order to quantify differences in expression. With the use of a fluorescence plate reader, a near 100 fold increase in expression for the optimized melittin construct was observed.Notes
TBASource
While the amino acid sequence for melittin was derived from Apis mellifica, the genomic sequence was altered by hand for optimization in E. coli. The mCherry sequence was obtained from| Sequence Annotation | Location | Component / Role(s) |
| Melittin Optimus mCherry Tag BBa_B1002 BBa_B0030 BBa_I13453 | 155,235 236,958 959,992 131,145 1,130 | feature/cds CDS tag feature/tag engineered_region feature/BioBrick feature/rbs ribosome_entry_site promoter feature/promoter |